BRIEF COMMUNICATIONS Histochemical Detection of Specific Isozymes of Myosin in Rat Ventricular Cells

نویسندگان

  • Andrea Weisberg
  • Saul Winegrad
  • Marianne Tucker
  • George McClellan
چکیده

A histochemical method for distinguishing isozymes of myosin in rat ventricles has been developed. The procedure involves preincubation in pH 10.5, which inhibits Ca-activated ATPase of the V3 isozyme but not the Vi isozyme of myosin. The specificity of the technique has been demonstrated by comparison of results in hearts from young euthyroid and hypothyroid rats, in which the predominant isozymes are, respectively, Vi and V3. The technique is capable of detecting as small a change in the relative amount of Vi as 15% of the total myosin. Isozymes appear to be uniformly distributed within each ventricular cell. There is only a small difference in the content of Vi among the cells in a ventricular chamber of hearts from young euthyroid and hypothyroid rats, but in the period of rapid transition of isozyme content after thyroidectomy, there is considerable heterogeneity of Vi concentration among the cells. The functional implications of the mixture of isozymes is discussed. (Circ Res 51: 802-809, 1982)

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تاریخ انتشار 2005